Journal: bioRxiv

Article Title: Pathological TDP-43 filaments accumulate at synapses and cause synaptic dysfunction

doi: 10.64898/2026.01.27.701787

Figure Lengend Snippet: A. Immunoblot of synaptosome isolation fractions (s, supernatant; cp, cell pellet; cyt, cytosolic; syn, synaptosomes) from mouse primary cortical neurons incubated with (+ filaments) and without (- filaments) FTLD-TDP Type A patient brain-derived TDP-43 filaments for 3 d, probed with antibodies against postsynaptic density protein 95 (PSD95, top), pS409/410 TDP-43 (pTDP-43, middle) and TDP-43 (bottom). The arrows indicate full-length (FL) TDP-43 and C-terminal fragments (CTFs) of TDP-43. B. Denoised tomographic slices of synaptosomes from mouse primary cortical neurons incubated with FTLD-TDP Type A patient brain-derived TDP-43 filaments for 3 d. TDP-43 filaments (magenta arrows), example synaptic vesicles (cyan arrows) and the postsynaptic density (yellow arrow) are indicated. C and D. Denoised tomographic slices showing TDP-43 filaments (magenta arrows). making contacts (green arrows) with synaptic vesicles (cyan arrows) (C) and the presynaptic plasma membrane (yellow arrows) (D) . B-D. Scale bars, 50 nm.

Article Snippet: The following primary antibodies were used: Anti-phospho-serine 409 and 410 TDP-43 (Cosmo-Bio, TIP-PTD-M01; 1:1,000); anti-mouse TDP-43 residues S379-S387 (in-house, 1:400); anti-class III β-tubulin (Biolegend, 801202; 1:200); anti-class III β-tubulin (Abcam, ab6046, 1:200); anti-bassoon (Merk, ABN255; 1:250); anti-postsynaptic density protein 95 (NeuroMab, 75028; 1:1,000); anti-microtubule-associated protein 2 (Abcam, ab5392; 1:500); anti-neuronal nuclei (Abcam, EPR12763; 1:200); anti-early endosome antigen 1 (BD Biosciences, 610457; 1:200); anti-clathrin (Abcam, ab21647; 1:200), anti-hook microtubule tethering protein 3 (Sigma-Aldrich, SAB1401891; 1:100); anti-chaperonin-containing T-complex subunit 5 (Proteintech, 11603-1-AP; 1:100); anti-DnaJ homolog subfamily A member 2 (Proteintech, 12236-1-AP; 1:200); anti-14-3-3 (Origene, TA327381; 1:100); anti-microtubule associated protein 1 light chain 3B (Cosmo-bio, CACCTB-LC3-2-IC; 1:50); and anti-proteasome 20S subunit β4 PSMB4 (ThermoFisher, A303-819A; 1:50).

Techniques: Western Blot, Isolation, Incubation, Derivative Assay, Clinical Proteomics, Membrane

Journal: Acta neuropathologica

Article Title: Brain regional correlation of amyloid-β with synapses and apolipoprotein E in non-demented individuals: potential mechanisms underlying regional vulnerability to amyloid-β accumulation.

doi: 10.1007/s00401-013-1086-9

Figure Lengend Snippet: Fig. 3 Strong regional associations between Ab and PSD95 or apoE. a PSD95 levels in each brain area are plotted against Ab40 levels in TBS fraction in each brain area. b ApoE levels in TBS-TX fraction in each brain area are plotted against Ab40 levels in TBS-TX fraction in each brain area. c ApoE levels in TBS fraction in each brain area are plotted against Ab40 levels in GuHCl fraction in each brain area. Values are median with 25 and 75 percentiles. Correlation coefficient (r) and p value were acquired by Spearman rank correlation test. GuH GuHCl

Article Snippet: The recombinant human PSD95 proteins (Novus Biologicals, Littleton, CO, USA) were used as standards.

Techniques:

Journal: Acta neuropathologica

Article Title: Brain regional correlation of amyloid-β with synapses and apolipoprotein E in non-demented individuals: potential mechanisms underlying regional vulnerability to amyloid-β accumulation.

doi: 10.1007/s00401-013-1086-9

Figure Lengend Snippet: Fig. 4 Strong regional associations among molecules related to Ab metabolism. a GFAP levels in each brain area are plotted against apoE levels in TBS fraction in each brain area. b PSD95 levels in each brain area are plotted against LRP1 levels in each brain area. c GFAP levels in each brain area are plotted against lactate levels in each brain area. Values are median with 25 and 75 percentiles. Correlation coefficient (r) and p value were acquired by Spearman rank correlation test

Article Snippet: The recombinant human PSD95 proteins (Novus Biologicals, Littleton, CO, USA) were used as standards.

Techniques:

Journal: Acta neuropathologica

Article Title: Brain regional correlation of amyloid-β with synapses and apolipoprotein E in non-demented individuals: potential mechanisms underlying regional vulnerability to amyloid-β accumulation.

doi: 10.1007/s00401-013-1086-9

Figure Lengend Snippet: Fig. 5 Correlation map based on the regional associations between Ab and molecules related with Ab metabolism. a Correlation map are depicted based on the regional associations among Ab40 in TBS fraction, apoE in TBS fraction, GFAP, PSD95, and LRP1. b Corre- lation map are depicted based on the regional associations among Ab40 in TBS-TX and GuHCl fraction, apoE in TBS fraction, GFAP, PSD95, and LRP1. Values indicate correlation coefficient (r). Red line indicates a positive association. Blue line indicates a negative association. Solid line indicates statistical significant associations observed between two molecules. Dashed line indicates no statistical significant associations between two molecules. Bold line indicates the strongest correlation between Ab40 and PSD95, and between Ab40 and apoE

Article Snippet: The recombinant human PSD95 proteins (Novus Biologicals, Littleton, CO, USA) were used as standards.

Techniques: